In this post, we present a pipeline for scRNA-seq analysis of lymphocytes (B-cells, T-cells and NK-cells) using the “census of immune cells” dataset from the Human Cell Atlas project. We will walk through the analysis step-by-step and mention the main differences between scRNA-seq and bulk RNA-seq. 1. Quality Control High quality libraries are considered asContinue reading “Single cell RNA-seq Analysis of Lymphocytes – How far it differs from Bulk RNA-seq Analysis?”

In this post, we present a visual approach for exploring genotypic data and performing genome-wide association and epistasis analysis using a simulated phenotype for the YRI (Yoruba in Ibadan, Nigeria) cohort from the International HapMap project. We start first with quality control process for SNPs and samples. 1. Quality Control Data for genome-wide association andContinue reading “Visual Guide to Genome Wide Association and Interaction Analyses using PLINK and R”